The powerplex y23 system allows ystr analysis of human forensic and database samples. We have created allelic ladders following international. Quick 2prtc thermal cycling use of the powerplex y23 system to amplify dna from storage card punches nstructions for use of products dc2305 and dc2320. The 5dye str multiplex amplifies 23 ystr loci, including two. Internal validation of the powerplex y23 amplification kit for use in forensic casework jessica tokarz bs1, scott milne bs2, sarah foster bs2, pamela staton phd1, kathleen press ms3 1marshall university, 1401 forensic science drive, huntington, wv 25701 2colorado springs metro crime laboratory, 705 s nevada avenue, colorado springs, co 80903. The 5dye str multiplex amplifies 23 ystr loci, including two rapidly mutating ystr loci. Validation and casework implementation of the promega.
Validation of powerplex y23 pcr amplification system for. Use your population samples file excel to start amova. A fast, sensitive and reliable ystr multiplex system for forensic and population genetic purpose article pdf available june 2016 with 392 reads how we. All primer pairs, with the exception of the se33 primer pairs, are identical in both systems. Protocols for dna amplification using the powerplex y23 system. The powerplex fusion system and all system components are manufactured in accordance with iso 18385. Powerplex y23, ampflstr yfiler, and two inhouse multiplexes, which together accounts for 27 different. Email promega technical services if you have questions on use of this system. However, if casework direct lysate is added, the volume should not exceed 15l. For the powerplex y23 system it is possible to add sample volumes of 17. This system coamplifies the loci d18s51, d21s11, th01, d3s58, penta e labeled with fluorescein. The volume of internal lane standard used in the loading cocktail can be adjusted to. Be sure to provide haplotypes only for markers you are going to use in your amova calculation. The y chromosome haplotype reference database yhrd is a computing platform, which allows acquisition, distribution, evaluation and interpretation of forensic dna datasets.
The powerplex y23 system is designed for use with the geneamp pcr system 9700 with a silver or goldplated silver sample block with max mode as the ramp speed. Rapid processing of swabs from casework samples using. Protocol for amplification and analysis of dna from swabs. Panels and bins text files are required for automatic assignment of genotypes using the genemapper.
Overview protocols specifications resources amplify 23 ystr loci in a single reaction the powerplex y23 system is a 23loci, 5color ystr multiplex. Visit the web site to verify that you are using the most current version of this technical manual. Protocol for amplification and analysis of extracted dna. Ystr analysis afdaa workshop june 2012 jack ballantyne, phd national center for forensic science university of central florida. Validation and implementation of the promega powerplex y23. Learn how to interpret results from ystr dna typing using the ychromosome haplotype reference database yhrd, a free resource. This change reduces the number of matrix standards required to use the powerplex systems and increases convenience for the end user. In the second sample, sequencing confirmed 8 and 11 alleles at d7s820. Power plex y 23 system markers source powerplex y23. Use of the powerplexr y23 system to amplify dna from. Importing powerplex y23 panels, bins and stutter text files into genemapper idx. This study focuses on the validation of the powerplex y23 system for use in. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 3 tmd035 revised 616 the powerplex y23 system provides all materials necessary to amplify ystr regions of human genomic dna, including a hotstart dna polymerase, which is a component of the powerplex y23 5x master mix.
Quick 2prtc thermal cycling use of the powerplex y23 system to amplify extracted dna instructions for use of products dc2305 and dc2320. Powerplexr y23 system for use on the applied biosystems. A casework pilot and case study jim thomson dna innovation manager, lgc science for a safer world. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Importing powerplex y23 panels and bins text files with genemapper. All necessary materials are provided to amplify str regions of human genomic dna, including a hotstart thermostable dna polymerase, which is a component of the powerplex fusion 5x master mix. Recently launched ystr kit, powerplex y23 pp y23 system is a.
The chemistry modifications did not necessitate any changes in the powertyper macros or in the panel and bin files for the genemapper id software. This fivecolor multiplex system is optimized for direct amplification of sample on fta cards. Internal validation of the powerplex y23 amplification kit. Ystr haplotype reference database yhrd is a searchable worldwide database of ystr haplotypes in various formats along with tools build upon their distribution.
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